The Phytochemical Compounds and the Antimicrobial Activity of Portulaca grandiflora Aqueous & Methanol Extracts in Iraq

Mahasen Abid –Ali Alkafaji; Neepal Imtair Al Garaawi; Fatima Karim Khudair

doi:10.53851/psijk.v2.i7.40-45

المؤلفون

Mahasen Abid –Ali Alkafaji Biology of Department, College of Education for Pure Science, University of Kerbala, Kerbala, Iraq
Neepal Imtair Al Garaawi Biology of Department, College of Education for Pure Science, University of Kerbala, Kerbala, Iraq
Fatima Karim Khudair Biology of Department, College of Education for Pure Science, University of Kerbala, Kerbala,

DOI:

https://doi.org/10.53851/psijk.v2.i7.40-45

الكلمات المفتاحية:

Portulaca grandiflora، gas chromatography–mass spectrometry، bioactive phytochemical، antibacterial activity، Proteus mirabilis، antifungal activity، Candida albicans

الملخص

Inhibitory activity against bacterial and fungal pathogens is one of the many diseases for which phytochemical compounds are useful in medicine. By using gas chromatography-mass spectrometry (GC-MS) on the floral parts of Portulaca grandiflora s in Iraq, several phytochemicals were extracted for this study. The study's objective is to determine the active substances found in the species' vegetative parts and evaluate their ability to inhibit pathogens. In the current study, various medical phytochemical compounds that have been isolated from Portulaca grandiflora from the floral parts that showed antifungal activity as Candida albicans and antibacterial as Proteus mirabilis. The phytochemicals of Portulaca grandiflora floral parts were exposed to gas chromatography–mass spectrometry (GC-MS) analysis. The results revealed the highest activity against fungal and bacteria. The study used six concentrations of the floral parts (5,10,20,40,60,80) mg/ml for both aqueous and methanolic extracts. The aqueous extract showed high inhibition against Candida fungi, It was (0.00mm, 0.00mm, 0.00mm, 0.00mm, 12.00mm, 22.00mm) respectively in the diameter of colonies Thus, the aqueous extract indicated high inhibition against bacteria Proteus mirabilis . It was (0.00mm, 0.00mm, 0.00mm, 10.00mm, 14.00mm, 18.00mm) separately in the diameter of colonies. The methanolic extracts of floral parts have lower inhibition rates compared to the aqueous extract.which was giving results against Candida fungi (0.00mm, 0.00mm, 4.00mm, 6.00mm, 10.00mm, 24.00mm) respectively in the diameter of colonies, so that in a result with the inhibition against bacteria Proteus mirabilis . It was (0.00mm ,3.00mm, 6.00mm,10.00mm, 15.00mm, 25.00mm) respectively in the diameter of colonies. The analysis of compounds to P. grandiflora floral parts by GC-MS showed the presence of five bioactive phytochemical compounds as: Benzaldehyde : Cyclohexene , 1-methyl-4-(1-methylethenyl)-: (S), Linalool : Naphthalene : and Cyclohexanol, 1-methyl-4-(1-methylethenyl)-, acetate.