First Report of Alternaria alternata Causing Leaf Spot on Orange (Citrus sinensis (L.) Osbeck) in Karbala Province, Iraq

Authors

  • Zainab L. Hameed Field Crop Department, Agriculture College, University of Kerbala, Karbala, Iraq

DOI:

https://doi.org/10.53851/psijk.v3.i9.88-91

Keywords:

Citrus sinensis, citrus leaf spot, Alternaria alternata, ITS-rDNA, fungal foliar disease

Abstract

During a field survey conducted in 2022 in citrus groves in Alhusaynia territory, Karbala Province, Iraq, leaf spot symptoms were observed on orange foliage. A fungal pathogen was regularly isolated from diseased leaves and identified as Alternaria alternata based on colony and conidial morphology and internal transcribed spacer (ITS-rDNA; PX763615.1) sequence analysis. Pathogenicity was evaluated using a detached-leaf assay, and typical symptoms were reproduced on inoculated leaves, followed by re-isolation of the same fungus. This investigation is the first documented occurrence of A. alternata associated with leaf spot on orange in Karbala Province, Iraq

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Published

2026-03-31

How to Cite

L. Hameed , Z. (2026). First Report of Alternaria alternata Causing Leaf Spot on Orange (Citrus sinensis (L.) Osbeck) in Karbala Province, Iraq . Pure Sciences International Journal of Kerbala, 3(9), 88–91. https://doi.org/10.53851/psijk.v3.i9.88-91