Molecular identification of antibiotics resistance genes of pathogenic bacteria isolated from different clinical sources

Kerbala University- College of sciences- Department of biology

الملخص

Most of the acquired antibacterial resistance can be obtained by mutations occurring in genes due to certain physical and chemical factors. These mutations are present in chromosomal gene and  plasmids , among other types of mobile genetic elements. Aim of the current study is to determine the  resistance genes in isolated pathogenic bacteria and their effectiveness in resisting pathogenic bacteria to antibiotics. In the present study, Samples from patients suffering from burns, wounds and urinary tract infection were collected . These samples were cultured on the proper media and colonies were identified by different biochemical tests. Identified pathogenic bacteria were applied to investigate their resistance against wide range of antibiotics using VITEK system. PCR technique was used to detect the antibiotics resistant genes among bacterial isolates. Results showed that K. pneumoniae carried all tested genes. However, tem and shv genes were absent in St. aureus, E. coli and P. aeruginosa. It can be concluded that all tested bacteria were dangerous due to the antibiotics resistance activity but K. pneumoniae were the most dangerous.