Histochemical study for Effect of Asparagus officinalis L. roots extract on ovarian histology in female rat with Polycystic Ovary Syndrome

Abstract

Polycystic ovarian syndrome (PCOS) is characterized by several symptoms, such as hirsutism hyperandrogenism, amenorrhea, anovulation, sterility, as well as metabolic and endocrine disorders. This study was conducted to evaluate the efficacy of an extract from the roots of Asparagus officinalis L. in treating female rats with induced polycystic ovarian syndrome. In this study, the impact of 400mg/kg of asparagus officinalis L. roots extract on the polycystic ovarian syndrome was examined over a period of 28 days in order to scrutinize the effects of asparagus officinalis L. roots extract on letrozole-induced polycystic ovary syndrome.

Thirty female albino rats were chosen. They were divided into five groups. Group 1 Control, Group 2 asparagus officinal is Extract 400 mg/ kg for 28 days, Group 3 is the PCOS inducer. Rats were given letrozole (1 mg/kg) orally for 28 days in a 0.5% Carboxy-Methylcellulose (CMC) solution. Group 4 is a treatment group in which the animals were dosed with letrozole for 28 days. After that, they were dosed with asparagus root extract for another 28 days, and Group 5 is the preventive group, which received asparagus root extract for 28 days. Then, it was dosed with letrozole for the same period. The Trichrome stain showed results low in stroma and collagen fibers of the structure ovaries in all groups, including control except the PCOS group that indicates a high presence of collagen. In addition to the number of primary, antral, and graphene follicles increased considerably. The treatment and preventative groups showed fewer cystic follicles than the PCOS group. The findings reveal decrease in collagen fibers by asparagus root extract. Therefore, it speeds up oogenesis and early cell divisions.