D Detection of Some Antibiotic Resistance of beta lactam and flouroquinolines Gene Among Coliform Bacteria isolated from Al-Hussainiya river

University of kerbala , College of science, Iraq, Corresponding author

Abstract

    Background: Coliform bacteria  are  the most important bacterial contaminate the water bodies.

 Objectives: the present study aimed to detect  antibiotics resistance and biofilm formation activities among coliform bacteria isolated from different places on Al-Hussainiya river.

Method :- During a period of July 2023 to February 2024,  18 samples were collected from three different places ( Baron, Al-Atishi area and the white arch) on Al-Hussainiya river in summer and winter (9 samples in summer and 9 samples in winter  for isolation of coliform. Most probable number (MPN) methods were used for evaluated the numbers of coliform bacteria during two seasons. The isolated coliform were further identified using Biochemical tests and Vitek 2 technique. PCR method was used to detect some genes including SHV, TEM, CTX, qur-A and Aac(6) Ib in 15  isolates of coliform during summer and winter. The current data showed that the numbers of coliform were significantly higher in summer in the white arch and Baron hotel. In contrast coliform appeared to be significantly lower in winter compared to summer in Al-Atishi point. Two way anova showed significant differences between stations and seasons (P≤0.05).

The results :- of the antibiotics resistance test showed isolates of E.coli , Klebsiella and Enterobacter spp resistant to all tested types of antibiotic during summer, where's  in winter season only E. coli were resistance to a few types of antibiotics.. The results of molecular identification showed that _SHV, _TEM, _CTX-M qur-A, Aac (6)Ib, bla_SHV, bla_TEM and bla _CTX-M genes were detected in 3 isolates of E. coli during winter only. However, these genes were not identified in Klebsiella and Enterobacter spp. In contrast, no isolates were reported to have any genes during summer season. In addition, most isolates of coliform were found to be biofilm producer bacteria.

Conclusion: coliform bacteria were ability   to biofilm formation and  their resist or sensitive for antibiotics.