Preparation, Characterization and Spectrophotometric determination of Chromium ion via Azotitation reaction and its application

Abstract

The reagent of Azo compound Ethyl4-(5-acetyl-2,3,4-trihydroxyphenyl) diazenyl benzoate (EATDB), was prepared ,characterized, and examined  by using  UV-Vis., FT-IR, G.C/Mass spectrum, ¹HNMR spectrum and ¹³C NMR. For a quick determination of Cr (III) ion employing (EATDB) as spectrophotometer reagent, which a sensitive and selective spectrophotometric approach is suggested. Chromium(III) and this reagent react instantly at (max=573nm) and pH=(8) to create prepull complexes with a mole ratio, Job's method and Mollard's method 1: 2 (Cr⁺³: EATDB) for Cr (III), the absorbance is stable for more than 24 hours. With molar absorptivity ( Ɛ) = (0.935x10² L.mol^-1.cm^-1 and a limit of detection of 0.203 g.ml^-1 and a limit of quantification of 0.669 g.mL , Beer's law is observed in the range of (1-100) g.mL^-1. The relative standard deviation was (0.742%). Chromium (III) in pharmaceuticals (drugs containing chromium and niacin) can be determined satisfactorily using this approach.The most interferences were brought on by Ni(II), Zn(II), Co(II),Fe(III),Pb(II), and  Cd(II) and suitable masking agent were used.