Evaluation of the inhibitory activity of some plant extracts against Staphylococcus aureus and Klebsiella pneumoniae clinically isolated from patients with tonsillitis.

Abstract

The inhibitory activity of hot aqueous extract of Camellia sinensis, Syzygium aromaticum, glubara  Glycyrrhizin and Cinnamomum verum at concentrations (100, 80, 60, 40, 20, 10) mg/ml was investigated against 6 isolates of Staphylococcus aureus and 7 isolates of Klebsiella pneumoniae. Previously from patients with tonsillitis in the holy city of Karbala, these isolates are characterized by multidrug resistance (MDR) and has a strong ability to build biofilm.

  The results showed that the green tea extract had a higher inhibition efficiency, with an inhibition diameter of 19.67 mm at a concentration of 100 mg / ml of the extract against S. aureus, while the highest inhibition diameter was 14.5 mm against K. pnuemoniae at a concentration of 100 mg / ml of the extract compared to the rest. The remaining plant extracts, and the rest of the extracts did not show any inhibitory activity against K.pnuemoniae bacteria isolates.

The Minimum Inhibitory Concentration (MIC) value for green tea extract was determined at a concentration of 8 mg/ml against S. aureus and 36 mg/ml against K.pneumoniae.

The cytotoxicity of green tea extract was also studied, as the percentage of toxicity was 8%, and it was found that the results were good, and there was no hemolytic activity against human red blood cells.